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In this text, leading researchers discuss the role that inducible proteins play in cellular metabolism and the approaches being used to delineate the underlying molecular events that lead to their synthesis. The use of both classical methods such as protein purification and characterization, as well as molecular methods such as the use of antisense DNA to down-regulate the synthesis of specific target enzymes, as approaches to investigate the role particular enzymes play in cellular metabolism are discussed. Other chapters discuss molecular approaches to the study of gene expression, the identification and characterization of trans-acting transcription factors and attempts to dissect other parts of the signal transduction pathway by the search for pathway mutants.
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Plants are able to respond and adapt to changing environmental and endogenous signals by the induction of the synthesis of specific proteins which act to modify cellular metabolism. Environmental signals are diverse and include, for instance, nutrient availability, temperature, light, anaerobiosis, and pathogen attack amongst others, whilst endogenous signals include changes in the level of plant growth regulators. In this text, leading researchers discuss the role that inducible proteins play in cellular metabolism, and the approaches being used to delineate the underlying molecular events which lead to their synthesis. The use of both classical methods, such as protein purification and characterisation, as well as molecular methods, such as the use of antisense RNA to down-regulate the synthesis of specific target enzymes, are considered as approaches to investigate the role particular proteins play in cellular metabolism. Other chapters discuss molecular approaches to the study of gene expression, the identification and characterisation of trans-acting transcription factors and attempts to dissect other parts of the signal transduction pathway by the search for pathway mutants. This timely review volume will be of great value and interest to final year undergraduates, graduate students and researchers in the fields of plant biochemistry and molecular biology. SOCIETY FOR EXPERIMENTAL BIOLOGY SEMINAR SERIES: 49 INDUCIBLE PLANT PROTEINS: THEIR BIOCHEMISTRY AND MOLECULAR BIOLOGY SOCIETY FOR EXPERIMENTAL BIOLOGY SEMINAR SERIES A series of multi-author volumes developed from seminars held by the Society for Experimental Biology. Each volume serves not only as an introductory review of a specific topic, but also introduces the reader to experimental evidence to support the theories and principles discussed, and points the way to new research. 2. Effects of pollutants on aquatic organisms. Edited by A.P.M. Lockwood 6. Neurones without impulses: their significance for vertebrate and invertebrate systems. Edited by A. Roberts and B.M.H. Bush 8. Stomatal physiology. Edited by P. G. Jarvis and T.A. Mansfield 10. The cell cycle. Edited by P.C.L. John 11. Effects of disease on the physiology of the growing plant. Edited by PC Ayres 12. Biology of the chemotactic response. Edited by J. M. Lackie and P. C. Williamson 14. Biological timekeeping. Edited by J. Brady 15. The nucleolus. Edited by E.G. Jordan and C.A. Cullis 16. Gills. Edited by D.F. Houlihan, J.C. Rankin and T.J. Shuttleworth 17. Cellular acclimatisation to environmental change. Edited by A.R. Cossins and P. Sheterline 19. Storage carbohydrates in vascular plants. Edited by D.H. Lewis 20. The physiology and biochemistry of plant respiration. Edited by J.M. Palmer 21. Chloroplast biogenesis. Edited by R.J. Ellis 23. The biosynthesis and metabolism of plant hormones. Edited by A. Crozier and J'.R. Hitlman 24. Coordination of motor behaviour. Edited by B.M.H. Bush and F. Clarac 25. Cell ageing and cell death. Edited by I. Davies and D.C. Sigee 26. The cell division cycle in plants.