E-Book Overview
Since the publication of the bestselling second edition of John Walker's widely acclaimed <EM>Protein Protocols Handbook, there have been continual methodological developments in the field of protein chemistry. This greatly enhanced third edition introduces 57 critically important new chapters, as well as significantly updating the previous edition's tried-and-true methods. Although the timely new chapters are spread throughout all of the book, the vital section on post-translational modifications has been expanded most to reflect the increasing importance of these modifications in the understanding of protein function.
Each readily reproducible method follows the highly praised format of the <EM>Methods in Molecular Biology™ series, offering a concise summary of its basic theory, a complete materials list, a step-by-step protocol for its successful execution, and extensive notes on avoiding pitfalls, or on modifying the method to function within your own experimental circumstances. The expert authors of each chapter have demonstrated a hands-on mastery of the methods described, fine-tuned here for optimal productivity.
Comprehensive, cutting-edge, and highly practical, <EM>The Protein Protocols Handbook, Third Edition is today's indispensable benchtop manual and guide, not only for all those new to the protein chemistry laboratory, but also for those established workers seeking to broaden their armametarium of techniques in the urgent search for rapid and robust results.
E-Book Content
The Protein Protocols Handbook SPRINGER PROTOCOLS HANDBOOKS For other titles published in this series, go to www.springer.com/series/8623 The Protein Protocols Handbook THIRD EDITION Edited by John M. Walker University of Hertfordshire, Hatfield, UK Editor John M. Walker University of Hertsfordshire Hatfield, Hertsfordshire UK ISBN 978-1-60327-474-6 DOI 10.1007/978-1-59745-198-7 e-ISBN 978-1-59745-198-7 Library of Congress Control Number: 2009932604 ã Humana Press, a part of Springer Science+Business Media, LLC 2009 All rights reserved. This work may not be translated or copied in whole or in part without the written permission of the publisher (Humana Press, c/o Springer Science+Business Media, LLC, 233 Spring Street, New York, NY 10013, USA), except for brief excerpts in connection with reviews or scholarly analysis. Use in connection with any form of information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed is for-bidden. The use in this publication of trade names, trademarks, service marks, and similar terms, even if they are not identified as such, is not to be taken as an expression of opinion as to whether or not they are subject to proprietary rights. While the advice and information in this book are believed to be true and accurate at the date of going to press, neither the authors nor the editors nor the publisher can accept any legal responsibility for any errors or omissions that may be made. The publisher makes no warranty, express or implied, with re-spect to the material contained herein. Printed on acid-free paper Springer is part of Springer Science+Business Media (www.springer.com) Preface Since the second edition of this book was published in 2002 there have, of course, been continual methodological developments in the field of protein chemistry. Consequently, for this third edition, I introduced 57 chapters/protocols not present in the second edition, significantly updated a number of chapters remaining from the second edition and increased the overall length of the book from 164 to 208 chapters. The new chapters are generally spread across the various sections of the book, but in particular we have expanded the se