Developmental Biology Protocols, Volume I (methods In Molecular Biology Vol 135)

E-Book Overview

Drawing on state-of-the-art cellular and molecular techniques as well as new and sophisticated imaging and information technologies, this comprehensive, three-volume collection of cutting-edge protocols provides readily reproducible methods for studying and analyzing the events of embryonic development. Volume 1 (ISBN: 089603-574-3) contains techniques for establishing and characterizing several widely used experimental model systems, for the study of developmental patterns and morphogenesis, and for the examination of embryo structure and function. There are also step-by-step methods for the analaysis of cell lineage, the production and use of chimeras, and the experimental and molecular manipulation of embryos, including the application of viral vectors. Volume 2 (ISBN: 0-89603-575-1) describes state-of-the-art methods for the study of organogenesis, the analysis of abnormal development and teratology, the screening and mapping of novel genes and mutations, and the application of transgenesis, including the production of transgenic animals and gene knockouts. No less innovative, Volume 3 (ISBN: 0-89603-576-X) introduces powerful techniques for the manipulation of developmental gene expression and function, the analysis of gene expression, the characterization of tissue morphogenesis and development, the in vitro study of differentiation and development, and the genetic analysis of developmental models of diseases. Highly practical and richly annotated, the three volumes of Developmental Biology Protocols describe multiple experimental systems and details techniques adopted from the broadest array of biomedical disciplines.

E-Book Content

Methods in Molecular Biology TM TM VOLUME 135 Developmental Biology Protocols Volume I Edited by Rocky S. Tuan Cecilia W. Lo HUMANA PRESS Developmental Biology Protocols M E T H O D S I N M O L E C U L A R B I O L O G Y™ Developmental Biology Protocols Volume I Edited by Rocky S. Tuan Thomas Jefferson University, Philadelphia, PA and Cecilia W. Lo University of Pennsylvania, Philadelphia, PA Humana Press Totowa, New Jersey To Chuck and our parents © 2000 Humana Press Inc. 999 Riverview Drive, Suite 208 Totowa, New Jersey 07512 All rights reserved. No part of this book may be reproduced, stored in a retrieval system, or transmitted in any form or by any means, electronic, mechanical, photocopying, microfilming, recording, or otherwise without written permission from the Publisher. Methods in Molecular Biology™ is a trademark of The Humana Press Inc. All authored papers, comments, opinions, conclusions, or recommendations are those of the author(s), and do not necessarily reflect the views of the publisher. This publication is printed on acid-free paper. ∞ ANSI Z39.48-1984 (American Standards Institute) Permanence of Paper for Printed Library Materials. Cover design by Patricia F. Cleary Cover illustration taken from Fig. 1 in Chapter 20 and is described on page 205 of this volume. For additional copies, pricing for bulk purchases, and/or information about other Humana titles, contact Humana at the above address or at any of the following numbers: Tel.: 973-256-1699; Fax: 973-256-8341; E-mail: [email protected]; or visit our Website: http://humanapress.com Photocopy Authorization Policy: Authorization to photocopy items for internal or personal use, or the internal or personal use of specific clients, is granted by Humana Press Inc., provided that the base fee of US $10.00 per copy, plus US $00.25 per page, is paid directly to the Copyright Clearance Center at 222 Rosewood Drive, Danvers, MA 01923. For those organizations that have be
You might also like

Methods In Enzymology, Vol. 371: Rna Polymerases And Associated Factors, Part D
Authors: Sankar L. Adhya , Susan Garges (Eds.)    174    0


Industrial Fermentations
Authors: Paul W. Allen    145    0


The Gale Encyclopedia Of Genetic Disorders
Authors: Stacey Blachford    172    0



Rna-ligand Interactions, Part B
Authors: Melvin I. Simon , Daniel W. Celander , John N. Abelson    138    0


Flavoprotein Protocols
Authors: Steven K. Chapman , Graeme A. Reid    194    0


Mitochondrial Dna. Methods And Protocols
Authors: William C. Copeland    140    0


Protein Purification Protocols
Authors: Paul Cutler    141    0


Cryopreservation And Freeze-drying Protocols
Authors: Day J.G. , McLellan M.R.    161    0


Molecular Cytogenetics. Protocols And Applications
Authors: Yao-Shan Fan    165    0