sourcebook THE
A Handbook for Gel Electrophoresis
sourcebook THE
A Handbook for Gel Electrophoresis
Preface Genomics. Functional Genomics. Proteomics. Metabolomics. Molecular Biology. Biochemistry. Biology.
The names used to define areas of research in the life sciences are continually changing, but many of the basic questions researchers ask remain the same. Thus, many of the techniques they use remain the techniques that have been applied for many years. Techniques such as separation of nucleic acids by agarose gel electrophoresis and separation of proteins by polyacrylamide gel electrophoresis. Marine Colloids. FMC BioProducts. BMA. BioWhittaker Molecular Applications. Cambrex.
Our name has also changed over the years. But our high quality products and our commitment to our customers have not. For over 35 years we have supplied the research community with the best agarose products in the world and enabled nucleic acid separation, detection, modification and recovery. Those years of experience led to the development of the original The Sourcebook – “everything you could possibly want to know about agarose.” This new edition of The Sourcebook expands coverage to include information about protein separation and detection. You can still find the information and techniques necessary to get the maximum performance from our SeaKem®, NuSieve® and MetaPhor® Agarose products for DNA electrophoresis, including the latest information on staining. In addition, you will find the same comprehensive information and techniques for RNA electrophoresis, as well as for separation of nucleic acids in polyacrylamide gels. We’ve expanded the section on special applications of agarose, especially in cell culture. Plus, new to this edition of The Sourcebook, are sections covering the separation of proteins by polyacrylamide slab gels, IEF gels, and agarose gels. In each case, thorough information is provided on preparation, loading, running and detection. In keeping with our original Sourcebook, the information is presented in easy to access tables and charts. This manual can also be used at the bench as you perform your experiments. The step-by-step protocols are easy to follow and we include recipes for all the buffers used in this manual. We hope this becomes one of your preferred resources for all your electrophoretic separation needs. We look forward to and welcome your comments. Please contact us with your comments at:
[email protected]
sourcebook THE
A Handbook for Gel Electrophoresis Table of Contents I. Preparation of Agarose Gels
Selecting the Appropriate Agarose . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8 How to Select the Appropriate Agarose . .