E-Book Overview
A comprehensive and up-to-date set of basic and advanced methods that illuminates the principles of molecular diagnostics and allows researchers to establish assay systems tailored to their specific needs. This unique collection has been thoroughly updated and expanded and covers the latest strategies for identifying novel diagnostic markers, epitope mapping, and the production and purification of recombinant antigens and their use as diagnostic reagents in immunological assays. This new edition places significant emphasis on bioinformatics and proteomics and the characterization and modification of proteins. It also includes promising techniques based on recent developments in antibody engineering and on the use of biosensors. Rather than focusing on particular infectious agents, this book focuses on the techniques that can be used and tailored to accomplish any kind of molecular diagnostic application in the clinical laboratory, thus making it an essential book for all laboratory disagnosticians.
E-Book Content
1 Identification of Novel Diagnostic Markers by Differential Display Peng Liang, Feilan Wang, Weimin Zhu, Robert P. O’Connell, and Lidia Averboukh 1. Introduction Accurate and early diagnosis of a disease state such as a viral infection, or in a more complicated situation cancer, means live saving because proper medical interventions can be applied in a timely manner before it is too late to treat the disease. Thus it is crucial that good diagnostic markers for any relevant diseasescan be obtained. The markers can be in the form of DNA such as yirai integration or chromosomal DNA aberrations including deletions, translocations, and point mutations. As a result, these genetic abnormalities, in turn, may lead to altered gene expressions, such as new genes being turned on. Therefore, the markers can also be in the form of mRNAs or their protein products. DNA-based dia