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Assembles detailed protocols designed exclusively for clinical applications, often described by the scientists who developed or refined them in their own laboratories. They furnish new and more powerful assays for many routine serum and blood tests now regularly performed in clinical laboratories, including serum and urine proteins; hemoglobin separation; and detecting CSF proteins, lipoproteins, myoglobin, cryoglobulins, and cathepsin D. The protocols for DNA analysis include double-stranded DNA analysis, the prenatal diagnosis of Down's syndrome, Rh D/d genotyping, identifying the mutated p53 oncogene, and detecting microsatellite instability in cancer.
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Clinical Applications of CE
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1 Clinical Applications of Capillary Electrophoresis Margaret A. Jenkins
1. Introduction Capillary electrophoresis (CE) is a new and innovative technique that separates charged or uncharged molecules in a thin buffer-filled capillary by the application of a very high voltage. Separations by CE are extremely fast: Some are achieved in less than 5 min, with reproducibility studies often showing coefficient of variation (CVs) of $5 per concentrator), and the time saved (approx 30 min) by not concentrating the urine specimen. 3. The urine buffer vial on the instrument in position 55 is able to be used for 2 wk, providing there is no buffer depletion. The 0.1 M NaOH and water vials are changed twice a week. 4. Several automated urine protein methods, such as Coomassie or benzethonium chloride, may underestimate the total protein if Bence Jones protein is present.
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Jenkins
Fig. 3. Capillary electropherogram of urine with a total protein of 8.51 g/L. The double banded Bence Jones band which quantitated at 7.0 g/L, was shown by immunofixation to be caused by free κ light chains. Electrophoretic conditions as in Fig. 1. However, if