E-Book Overview
The critically acclaimed laboratory standard for forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerlyawaited, frequently consulted, and praised by researchers and reviewers alike. More than 250 volumes have been published (all of them still in print) and much of the material is relevant even today - truly an essential publication for researchers in all fields of life sciences.Key Features* Includes descriptions of functional, structural, kinetic, and genetic methods for analyzing major enzymes of DNA replication* Describes strategies for studying interactions of these proteins during replication* Provides comprehensive descriptions of uses of prokaryotic and eukaryotic crude in vitro replication systems and reconstitution of such systems from purified proteins* Includes methods for analyzing DNA replication in vivo
E-Book Content
Preface The increasing relevance of studies of D N A replication and D N A repair to the understanding of human genetic disease, cancer, and aging is bringing growing numbers of investigators into this field. The rich legacy of past studies of the enzymology of these processes has already had wide impact on how modern biological research is conducted in that it provided the roots for the whole field of genetic engineering. The work of the biochemist in characterizing these complex reactions is still far from done, however, since we are still short of the mark of being able to use our knowledge to prevent the devastating aberrations caused by failures of faithful copying of the genome by the self-editing D N A replication and repair apparatus. Past study of the enzymes involved in D N A replication has given rise to a number of highly refined approaches to defining their individual enzymatic mechanisms and how they interact to carry out the process