E-Book Overview
Advances in our understanding of biological mechanisms have frequently been associated with the development of new techniques. In situ hybridization is a classic case of just such an advance. The technique effectively combines histochemistry with molecular biology and enables the rapid analysis of the distribution of RNA, or DNA, in the tissues. The information gained from this has caused something of a revolution in our understanding of developmental biology, since a fundamental aspect of development is the spatial and temporal expression of genes. In addition the technique has found application in the field of medicine, where it is giving new insights into the functioning of healthy tissues and the diagnosis and study of diseases. This book brings together contributions from leaders in the application of this technique and will help the reader review and be guided through the various options and variations of the technique.
E-Book Content
SOCIETY FOR EXPERIMENTAL BIOLOGY SEMINAR SERIES: 40
In situ hybridisation: application to developmental biology and medicine
SOCIETY FOR EXPERIMENTAL BIOLOGY SEMINAR SERIES A series of multi-author volumes developed from seminars held by the Society for Experimental Biology. Each volume serves not only as an introductory review of a specific topic, but also introduces the reader to experimental evidence to support the theories and principles discussed, and points the way to new research. 1. Effects of air pollution on plants. Edited byT. A. Mansfield 2. Effects of pollutants on aquatic organisms. Editedby A. P. M. Lockwood 3. Analytical and quantitative methods. Edited by J. A. Meek andH. Y. Elder 4. Isolation of plant growth substances. Edited by J. R. Hillman 5. Aspects of animal movement. Edited by H. Y. Elder and E. R. Trueman 6. Neurones without impulses: their significance for vertebrate and invertebrate systems. Edited by A. Roberts and B. M. H. Bush 7. Development and specialisation of skeletal muscle. Edited by D. F. Goldspink 8. Stomatal physiology. Edited by P. G. Jarvis and T. A. Mansfield 9. Brain mechanisms of behaviour in lower vertebrates. Edited by P. R. Laming 10. The cell cycle. Edited by P. C. L. John 11. Effects of disease on the physiology of the growing plant. Edited by P. G. Ayres 12. Biology of the chemotactic response. Edited by J. M. Lackie and P. C. Williamson 13. Animal migration. Edited by D. 7. Aidley 14. Biological timekeeping. Edited by J. Brady 15. The nudeolus. Edited by E. G. Jordan and C. A. Cullis 16. Gills. Edited by D. F. Houlihan, J. C. Rank in and T. J. Shuttleworth 17. Cellular acclimatisation to environmental change. Edited by A. R. Cossins and P. Sheterline 18 Plant biotechnology. Edited by S. H. Manlell and H. Smith 19. Storage carbohydrates in vascular plants. Edited by D. H. Lewis 20. The physiology and biochemistry of plant respiration. Edited by J.M. Palmer
21. Chloroplast biogenesis. Editedby R. J. Ellis 22. Instrumentation for environmental physiology. Editedby B. Marshall and F. I. Woodward 23. The biosynthesis and metabolism of plant hormones. Editedby A. CrozierandJ. R. Hillman 24. Coordination of motor behaviour. Edited by B. M. H. Bush and F. Clarac 25. Cell ageing and cell death. Edited by I. Dalies and D. C. Sigee 26. The cell division cycle in plants. Editedby J. A. Bryant and D. Franeis 27. Control of leaf growth. Editedby N. R. Baker. W. J. Davies and C. Ong 28. Biochemistry of plant cell walls. Edited by C. T. Brett and J. R. Hillman 29. Immunology in plant science. Edited by T. L. Wang 30. Root development and function. Edited by P. J. Gregory, J. V. Lake and D. A. Rose 31. Plant canopies: their growth, form and function. Editedby G. Russell. B. Marshall and P. G. Jarvis 32. Developmental mutants in higher plants. Edited by H. Thomas and D. Grierson 33. Neurohormones in invertebrates. Editedby M. Thorndyk