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This book provides the most up-to-date review of the simian virus 40 (SV40) minichromosome as a model for the mammalian chromosome in studies of DNA replication. It focuses on disruption of DNA replication by anticancer drugs and DNA-damaging agents. There is a strong emphasis on the unique advantages of SV40 as an experimental system for the analysis of these classes of anticancer drug mechanisms. The new high-resolution gel electrophoresis methods for the analysis of SV40 DNA replication are covered in detail to aid readers in designing and interpreting similar experiments. Key Features* Presents unique advantages of SV40 as an experimental system for the study of classes of anticancer drugs* Details new high-resolution gel electrophoresis methods for the analysis of SV40 DNA replication* Provides details to help the reader design and interpret similar experiments
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BIOTECHNOLOGY INTELLIGENCE UNIT THE SV40 REPLICON MODELFORANALYSIS OF ANTICANCERDRUGS RobertM. Snapka Associate Professor of Radiology The Ohio State University Columbus, Ohio, U.S.A. KG. I~DESCoMP.,~Y Academic Press AUSTIN BIOTECHNOLOGY INTELLIGENCEUNIT THE SV40 REPLICON MODEL FOR ANALYSIS OF ANTICANCER DRUGS R.G. LANDES C O M P A N Y Austin, Texas, U.S.A. This book is printed on acid-free paper. Copyright 1996 9 by R.G. Landes Company and Academic Press, Inc. All rights reserved. No part of this book may be reproduced or transmitted in any form or by any means, electronic or mechanical, including photocopy, recording, or any information storage and retrieval system, without permission in writing from the publisher. Please address all inquiries to the Publisher: R.G. Landes Company 909 Pine Street, Georgetown, Texas, U.S.A. 78626 Phone: 512/863 7762; FAX: 512/863 0081 Academic Press, Inc. 525 B Street, Suite 1900, San Diego, California, U.S.A. 92101-4495 United Kingdom Edition published by Academic Press Limited 24-28 Oval Road, London NW1 7DX, United Kingdom International Standard Book Number (ISBN): 0-12-65360-9 Printed in the United States of America While the authors, editors and publisher believe that drug selection and dosage and the specifications and usage of equipment and devices, as set forth in this book, are in accord with current recommendations and practice at the time of publication, they make no warranty, expressed or implied, with respect to material described in this book. In view of the ongoing research, equipment development, changes in governmental regulations and the rapid accumulation of information relating to the biomedical sciences, the reader is urged to carefully review and evaluate the information provided herein. Library of Congress Cataloging-in-Publication Data The SV40 replicaon model for analysis of anticancer drugs / Robert M. Snapka p. cm. m (Biotechnology intelligence unit) Includes bibliographical references and index. ISBN 0-12-65360-9 (alk. paper) 1. SV40 (virus). 2. Viral genetics. 3. Antineoplastic agentsmResearchmMethodology. 4. DNA-drug interactions. I. Title. II. Series. [DNLM: 1. Polyomavirus macacaemgenetics. 2. Polyomavirus macacae--drug effects. 3. DNA Replicationmdrug effects. 4. Models, Genetic. QW 165.5.P2 W669s 1996] QR406.2.$56S64 1996 616.99'4061~dc20 DNLM/DLC 96-579 for Library of Congress CIP PUBLISHER'SNOTE R.G. Landes Company publishes six book series: Medical Intelligence Unit, Molecular Biolog7 Intelligence Unit, Neuroscience Intelligence Unit, Tissue Engineering Intelligence Unit, Environmental Intelligence Unit and Biotechnology Intelligence Unit. The authors of our books are acknowledged leaders in their fields and the topics are unique. Almost without exception, no other similar books exist on these topics. Our goal is to publish books in important and rapidly changing areas of bioscience for sophisticated researchers and clini