Comprehensive Natural Products Ii: Chemistry And Biology: Modern Methods In Natural Products Chemistry

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E-Book Overview

This work presents a definitive interpretation of the current status of and future trends in natural products-a dynamic field at the intersection of chemistry and biology concerned with isolation, identification, structure elucidation, and chemical characteristics of naturally occurring compounds such as pheromones, carbohydrates, nucleic acids, and enzymes. With more than 1,800 color figures, Comprehensive Natural Products II features 100% new material and complements rather than replaces the original work (©1999).* Reviews the accumulated efforts of chemical and biological research to understand living organisms and their distinctive effects on health and medicine * Stimulates new ideas among the established natural products research community-which includes chemists, biochemists, biologists, botanists, and pharmacologists * Informs and inspires students and newcomers to the field with accessible content in a range of delivery formats  

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E-Book Information

• Volume Info: Volume 9

• Year: 2,010

• Edition: 1

• Pages: 771

• Pages In File: 771

• Language: English

• Issue: до 2011-08

• Identifier: 0080453813,9780080453811

• Org File Size: 32,441,884

• Extension: pdf

• Toc: 1 CONAP_Contents_all_volumes......Page 1Overview and Introduction......Page 13Introduction......Page 16High-Performance Reversed-Phase Chromatography......Page 19High-Performance Normal-Phase Chromatography......Page 20High-Performance Hydrophilic Interaction Chromatography......Page 21High-Performance Size Exclusion Chromatography......Page 22Summary......Page 23Development of Analytical Method......Page 24Scaling Up to Preparative Chromatography......Page 30Multidimensional High-Performance Liquid Chromatography......Page 31Purification of Natural Products by MD-HPLC Methods......Page 32Operational Strategies for MD-HPLC Methods......Page 33Orthogonality of chromatographic modes......Page 34Compatibility matrix of chromatographic modes......Page 35Mono-, Sesqui-, Di-, and Triterpenes......Page 37Iridoids and Secoiridoids......Page 38Carotenoids......Page 40Saponins......Page 43Coumarins......Page 45Flavonoids and Isoflavonoids......Page 48Alkaloids......Page 50Conclusions......Page 52Abbreviations......Page 54References......Page 55Introduction......Page 61Protein Crystals......Page 62Obtaining Protein Crystals......Page 64Principles of Diffraction......Page 67Fourier Transforms......Page 70Diffraction as a Fourier Series......Page 72The Diffraction Experiment in Practice......Page 73Phasing Methods......Page 77Isomorphous Replacement......Page 78Anomalous Dispersion......Page 80Molecular Replacement......Page 84The Electron Density Map......Page 85Modifying the Electron Density Map......Page 86Interpreting the Electron Density Map......Page 87Model Building and Refinement......Page 89Modelling Disorder: Temperature Factor and Occupancy......Page 91Model Validation......Page 92An Example of a Crystal Structure Determination......Page 94References......Page 97Characterization by Circular Dichroism Spectroscopy......Page 100OR of Chiral Compounds